Figure 6.

Differential gene expression in the $\mathrm{\Delta }$CD25890 mutant. (A) To compare the transcriptome of the $\mathrm{\Delta }$CD25890 mutant with the wild type, both strains were grown for 10 h in SM broth, samples collected and RNA-seq performed. The graph represents the functional classes of the 165 genes that are up-regulated in the $\mathrm{\Delta }$CD25890 mutant based on the RNA seq data (the number represent the percentage of each functional class). See also Table S3. (B) Sporulation efficiency of the Ptet-sinRR’ alleles. The spore titer was determined 24 h following inoculation into SM sporulation medium supplemented with 50 nM of anhydrotetracycline. The heat resistant spore count by determining the cfu/mL obtained after treatment at 70ºC. The results shown are averages and standard deviations for three biological replicates.