Fig. 3. Hakai regulates Sxl alternative splicing and adult fly behavior.

a Diagram showing the alternative splicing event that produces male- or female-specific Sxl transcripts. The arrows indicate primers used for RT-PCR. b Sxl splicing was analyzed by RT-PCR using RNAs extracted from adult flies of indicated genotypes. Note the appearance of male-specific bands in Mettl3^SK2/Df, Mettl14^SK1, Hakai^SH2, Hakai^SH4 females (arrowheads). Male-specific bands: 2–3–4. Female-specific bands: 2–4. c Relative isoform quantification for Dsp1, CG8929, Aldh-III and fl(2)d by qPCR in control or m⁶A mutant flies. Hakai is involved in m⁶A-dependent splicing events. Data are presented as mean ± SD from three biological replicates. d yw flies have their wings properly folded. (e) Mettl3^SK2/Df, (f) Hakai^SH2, (g) Hakai^SH4 flies cannot fold their wings and exhibit a held-out wing phenotype (marked by the double arrows). The frequency of flies showing held-out wings were quantified in (h). i Flies of the indicated genotypes were tested for their flight abilities, and the number of flightless flies was quantified. All flies used from (c) to (i) were males. h–i Data are presented as mean ± SD from three to five biologically independent groups (h, n = 3, 4, 4, 3; i, n = 4, 4, 5, 5). Source data are provided as a Source Data file.