Fig. 5. Hakai does not mediate E-cadherin levels in wing discs.

a–a’ Lateral (a) and apical (a’) section of E-cadherin staining in wild-type wing-disc epithelia. b–b” E-cadherin is mainly localized in the cell membrane (b), while Hakai is mostly in the nucleus (b’) in the large tracheal cells. c–c” Expression of Hakai RNAi in the dorsal half of the disc (below the dashed line) using ap-Gal4 led to a strong reduction of Hakai (c’), but E-cadherin staining was not affected either laterally (c) or apically (c”). d–d” Lateral (d) and apical (d”) E-cadherin distribution is not changed in Hakai mutant clones marked by the absence of Hakai staining (d’), when crossing actin-Cas9 with U6-Hakai-sgRNA. The experiments in a-d” were repeated at least twice independently with similar results, and each time around 30 wing discs for any genotype were examined. Scale bars: 10 μm.