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. 2021 Apr 12;11:7953. doi: 10.1038/s41598-021-87217-8

Figure 4.

Figure 4

Administration of hAGP rescues renal fibrosis at day 14 after renal-IR in AGP KO mice. mRNA expression of (A) α-SMA and (B) Collagen 1a2 in kidney at day 14 after renal IR were determined by real-time PCR. (C) Hydroxyproline levels in the kidney were measured at 14 days after renal IR. (D) Representative photomicrographs of Picrosirius red-stained kidney sections and a semi-quantitative scoring analysis of picrosirius red-positive staining area at 14 after renal IR are shown. Lower panels are an enlarged image of the upper panel. Original magnification: × 200 (upper panels), × 400 (lower panels). Scale bars represent 100 μm. hAGP administration to AGP KO mice (2 mg/mouse/day, ip, from day 0 (before IR) to day 7) rescued IR-induced renal fibrosis. Date are expressed as the means ± SE (n = 4–8).