Fig. 1. In vivo time-course of neutrophil accumulation after poxvirus infection.

BALB/c mice were infected by intraperitoneal (i.p.) injection of 10⁷ PFU of NYVAC-C Δ3 virus and Ly6G⁺ CD11b⁺ neutrophils were measured at 6 h post infection (a). Percentages of neutrophils in peritoneal cavity (PEC) (b), spleen (c), and blood (d) at 0, 2, 6, and 10 h post infection from NYVAC-C Δ3 injected mice. Spleen tissue section and its magnification from NYVAC-C Δ3-infected mice. Ly6G⁺ neutrophils in green, CD3⁺ T cells in white, IgD⁺ B cells in red, and CD21/35⁺ cells in blue (e). Spleen tissue section from PBS and NYVAC-C Δ3-injected mice. Ly6G⁺ neutrophils in yellow, CD8⁺ T cells in purple (f). Distance of the nearest Ly6G⁺ cell from CD8⁺ cells. The nearest neighbor euclidean distance to Ly6G⁺ is expressed as probability density for each cell belonging to CD8⁺ clusters (g). Graphs show mean; each point represents an individual mouse. Data are representative of two independent experiments. *P < 0.05, ***P < 0.001, ****P < 0.0001.