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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Invest Dermatol. 2020 Oct 13;141(5):1286–1296.e4. doi: 10.1016/j.jid.2020.09.017

Figure 2. Responses to codeine by skin MCs are highly divergent across individuals with no correlation with allergic degranulation and inhibition by SCF.

Figure 2.

(a) HR was stimulated by C48/80 (10 µg/ml), SP (30 µM), codeine (50 µg/ml), and IgER CL (29C6 at 0.5 µg/ml) on skin MCs ex vivo; each dot corresponds to a single donor. The net release was pairwise plotted. R denotes the Spearman correlation coefficient. (b) Ex vivo skin MCs were pretreated with SCF for 15 minutes and then subjected to the different stimuli for 30 minutes. Note the opposite mode of regulation caused by MRGPRX2 ligands (codeine, C48/80, and SP) compared with IgER-triggered degranulation. ***P < 0.001, ****P < 0.0001. C48/80, compound 48/80; CL, crosslinking; HR, histamine release; IgER, IgE receptor; MC, mast cell; SCF, stem cell factor; SP, substance P.