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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Invest Dermatol. 2020 Oct 13;141(5):1286–1296.e4. doi: 10.1016/j.jid.2020.09.017

Figure 4. Codeine and C48/80 cause the activation of β-arrestin MRGPRX2 internalization, which depends on β-arrestin-1 in skin MCs.

Figure 4.

(a, b) HTLA cells were stimulated overnight with the indicated stimuli, and luminescence units were measured on a luminometer after substrate addition; mean ± SEM and n = 3. (c–e) Skin MCs were treated for 48 hours with ARRB1 selective, ARRB2 selective, or nontarget siRNA. (c) ARRB1 and ARRB2 mRNA expression. (d) MRGPRX2 cell surface expression after codeine (100 µg/ml), normalized to the matching unstimulated control (by net MFI, see Figure 1). Mean ± SEM and n = 6 for c and d. (e) Corresponding representative histograms showing MRGPRX2 expression after codeine triggering in ARRΒ-silenced versus control siRNA-treated cells. Color code is as explained in the figure. **P < 0.01, ***P < 0.001, ****P < 0.0001. C48/80, compound 48/80; h, hour; MC, mast cell; MFI, mean fluorescence intensity; siRNA, small interfering RNA.