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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Invest Dermatol. 2020 Oct 13;141(5):1286–1296.e4. doi: 10.1016/j.jid.2020.09.017

Figure 5. A combination of C48/80 with codeine does not increase MRGPRX2 internalization or degranulation over each stimulus alone.

Figure 5.

(a) Cultured skin-derived MCs were triggered by C48/80 (10 µg/ml), codeine (50 µg/ml), or C48/80 + codeine for different times. Cell surface MRGPRX2 expression was determined as in Figure 3. The data are presented as mean ± SEM of n = 8 individual experiments. **P < 0.01 for codeine versus c48/80, #P < 0.05 for codeine versus codeine + c48/80. (b) Ex vivo skin MCs were stimulated, and HR was measured as in Figure 1. C48/ 80, compound 48/80; h, hour; HR, histamine release; MC, mast cell; MFI, mean fluorescence intensity.