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. 2021 Apr 12;11:7873. doi: 10.1038/s41598-021-87020-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of mice with adipocyte-specific deletion of Angptl4. (a) mRNA expression of Angptl4 in liver, heart, quadriceps muscle (quad), gonadal white adipose tissue (gWAT), subcutaneous white adipose tissue (sWAT), and brown adipose tissues (BAT) from 8–12 week old female Angptl4^fl/fl (n = 8) and Angptl4^AdipoKO (n = 8) mice following a 6 h fast (mean ± SEM). (b–d) Fasting plasma triglyceride (b), non-esterified fatty acid (c), and blood glucose (d) levels in 8–12 week male and female Angptl4^fl/fl and Angptl4^AdipoKO mice following a 6 h fast (mean ± SEM; n = 7–11). (e, f) Fasted (6 h) female Angptl4^fl/fl (n = 8) and Angptl4^AdipoKO (n = 7) mice were injected intravenously with ³H-triglyceride–containing chylomicrons. (e) Clearance of radiolabel from the plasma 1, 5, 10, and 15 min after injection. Points represent percentage of radiolabel remaining in the plasma at the indicated time points compared to the 1 min time point (mean ± SEM). (f) Uptake of radiolabel after 15 min (% injected dose/g tissue) into the indicated tissues (mean ± SEM). (g) Heart, quadricep muscle (quad), gonadal white adipose tissue (gWAT), subcutaneous adipose tissue (sWAT), and brown adipose (BAT) tissue from fasted (6 h) female Angptl4^fl/fl and Angptl4^AdipoKO mice were harvested and lipase activity was measured (n = 6–9/group). (h) Liver was harvested from fasted (6 h) female Angptl4^fl/fl and Angptl4^AdipoKO mice (n = 8/group). Lipase activity was measured in the presence or absence of 1 M-NaCl to distinguish between hepatic and lipoprotein lipase. Bars show relative lipase activity in each tissue normalized to Angptl4^fl/fl (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 by t-test analysis (panels a–c, f, g) or repeated measures ANOVA (panel e).