Figure 2.

Body weights, fat mass, and metabolic phenotypes of Angptl4^fl/fl and Angptl4^AdipoKO mice. (a) Weekly body weights of male Angptl4^fl/fl and Angptl4^AdipoKO mice fed either a normal chow diet (NCD) or a high fat diet (HFD; 60% by kCal) for 12 weeks starting at 8 weeks of age (mean ± SEM; n = 12–19/group). (b) Tissues weights of heart, liver, kidney, quadriceps muscle (quad), epididymal white adipose (eWAT), subcutaneous white adipose tissue (sWAT), and brown adipose tissue (BAT) in male Angptl4^fl/fl and Angptl4^AdipoKO mice after 12 weeks on diet (mean ± SEM; n = 7–11group). (c) Lean muscle and fat mass of male Angptl4^fl/fl and Angptl4^AdipoKO mice as measured by NMR after 12 weeks on diet (mean ± SEM; n = 6–9/group). Plasma triglycerides (d), plasma non-esterified fatty acids (e), blood glucose (f), and liver triglycerides (g) of fasted (6 h) male Angptl4^fl/fl and Angptl4^AdipoKO mice at the end of 12 weeks on diet (mean ± SEM; n = 5–8/group). ###p < 0.001 for dietary differences by two-way ANOVA. *p < 0.05, **p < 0.01 for individual genotype-specific differences by multiple comparison after two-way ANOVA (Tukey correction).