Figure 3.

Chylomicron clearance and uptake and lipase activity in Angptl4^AdipoKO mice. (a, b) At the conclusion of 12 weeks of either normal chow diet (NCD) or high fat diet (HFD) Angptl4^fl/fl and Angptl4^AdipoKO male mice (n = 7–10/group) were fasted (6 h) and injected intravenously with ³H-triglyceride–containing chylomicrons. (a) Clearance of radiolabel from the plasma 1, 5, 10, and 15 min after injection. Points represent percentage of radiolabel remaining in the plasma at the indicated time points compared to the 1-min time point (mean ± SEM). *p < 0.05 by repeated measures ANOVA. (b) Uptake of radiolabel (% injected dose/g tissue) into the indicated tissues after 15 min (mean ± SEM). ##p < 0.01 for dietary differences by two-way ANOVA. *p < 0.05, ***p < 0.001 for individual genotype-specific differences by multiple comparison after two-way ANOVA (Tukey correction). (c) Heart, quadricep muscle (quad), epididymal adipose tissue (eWAT), subcutaneous adipose tissue (sWAT), and brown adipose (BAT) tissue from fasted (6 h) male Angptl4^fl/fl and Angptl4^AdipoKO mice were harvested and lipase activity was measured (n = 6–8/group). (d, e) Liver was harvested from fasted (6 h) male Angptl4^fl/fl and Angptl4^AdipoKO mice (d, NCD groups and e, HFD groups) (n = 6/group). Lipase activity was measured in the presence or absence of 1 M NaCl to distinguish between hepatic and lipoprotein lipase. Bars show relative lipase activity in each tissue normalized to Angptl4^fl/fl (mean ± SEM). #p < 0.05, ##p < 0.01, ###p < 0.001 for dietary differences by two-way ANOVA. ***p < 0.001 for individual genotype-specific differences by multiple comparison after two-way ANOVA (Tukey correction).