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. 2021 Apr 12;11:7873. doi: 10.1038/s41598-021-87020-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Chylomicron clearance and uptake and lipase activity in Angptl4^AdipoKO mice after chronic high-fat feeding. At the conclusion of 6 months of either normal chow diet (NCD) or high fat diet (HFD) Angptl4^fl/fl and Angptl4^AdipoKO male mice (n = 7–9/group) were fasted (6 h) and injected intravenously with ³H-triglyceride containing chylomicrons. (a) Clearance of radiolabel from the plasma 1, 5, 10, and 15 min after injection. Points represent percentage of radiolabel remaining in the plasma at the indicated time points compared to the 1-min time point (mean ± SEM). ***p < 0.001 by repeated measures ANOVA. (b) Uptake of radiolabel (% injected dose/g tissue) into the indicated tissues after 15 min (mean ± SEM). #p < 0.05, ##p < 0.01, ###p < 0.001 for dietary differences by two-way ANOVA. *p < 0.05, ***p < 0.001 for individual genotype-specific differences by multiple comparison after two-way ANOVA. (c) Heart, quadricep muscle (quad), epididymal adipose tissue (eWAT), subcutaneous adipose tissue (sWAT), and brown adipose (BAT) tissue from fasted (6 h) male Angptl4^fl/fl and Angptl4^AdipoKO mice were harvested and lipase activity was measured (n = 6–8/group). (d, e) Liver was harvested from fasted (6 h) male Angptl4^fl/fl and Angptl4^AdipoKO mice (d, NCD groups and e, HFD groups)(n = 6–9/group). Lipase activity was measured in the presence or absence of 1 M NaCl to distinguish between hepatic and lipoprotein lipase. Bars show relative lipase activity in each tissue normalized to Angptl4^fl/fl (mean ± SEM). #p < 0.05, ##p < 0.01, ###p < 0.001 for dietary differences by two-way ANOVA. *p < 0.05, **p < 0.01 ***p < 0.001 for individual genotype-specific differences by multiple comparison after two-way ANOVA.