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. 2021 Apr 12;11:7992. doi: 10.1038/s41598-021-86401-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression levels of key genes related to nitrogen absorption and assimilation including nitrate transporter (BjNRT), ammonium transporter (BjAMT), nitrate reductase (BjNR), nitrite reductase (BjNiR), glutamine synthetase (BjGS), and glutamate synthase (BjGOGAT), and glutamate dehydrogenase (BjGDH) in 7-days-old B. juncea L. roots after treatment with PEG (10%) and its combination with NO (100 µM) and ABA (10 µM) (nitrate agonists) for 96 h. Expression values were calculated relative to the expression of plants grown in control condition under normalization with actin gene (2^−ΔΔCT). Values are mean with CIs of three independent experiments, each including four biological replicates. *p < 0.05 compared with control indicates significant difference.