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. 2021 Apr 12;11:7862. doi: 10.1038/s41598-021-87329-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Flow diagram for the inclusion of study participants, samples, and medical records. Results from 119 participants were used for HPV DNA genotyping in first-void urine and cervical samples. Test results from high-risk HPV DNA positive women (in first-void urine and/or cervical smear, n = 93) were used to examine methylation marker performance; using either cytology results (CYTOLOGY ARM), or histology outcomes (HISTOLOGY ARM) as reference. ^aWhen unavailable at D0 (day of study visit, i.e. first-void urine collection), the HPV DNA genotyping, liquid based cytology (LBC), colposcopy, and/or histology results from D0 ± 3 months were included for data analysis instead. Thus, three additional HPV DNA genotyping results were included, as well as four LBC and colposcopy, and six histology results.