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. 2021 Apr 12;5:29. doi: 10.1038/s41698-021-00166-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Shown are bright-field microscopy images of PDTs and PDOs. Growth factors that comprise AO media were removed from AO media as indicated to determine which growth factors are indispensable for the growth of lung tumoroids and normal lung organoids. Representative images of the PDTs/PDOs in each condition at the passage number indicated in c are shown. Scale bar, 200 μm. b The growth of the tumoroids/organoids was quantified by measuring three independent areas of the tumoroid/organoid occupancy ratio in the images shown in a. Data are shown as mean ± SD. c Passage numbers of the PDTs and PDOs in the indicated media conditions are assessed to determine which condition are most suitable for culture. We noted that a number of the conditions slowed the growth of normal lung organoids though they were passaged over 10 times (depicted as *).