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. 2021 Apr 12;5:29. doi: 10.1038/s41698-021-00166-3

Fig. 5. AO media is superior to other media on the growth of lung tumoroids (PDTs) and normal lung organoids (PDOs).

Fig. 5

a Shown are bright-field lignant effusion of lung cancer with acquired resincluding ones from three different laboratories (Kim-Jang, Shi-Tsao and Endo-Inoue) as indicated. Representative images of the PDTs/PDOs in each medium at the passage number indicated in c are shown. Scale bar, 200 μm. b The growth of the tumoroids/organoids was quantified as described in Fig. 4b. Data are shown as mean ± SD. c Passage numbers of the PDTs and PDOs in the indicated media are assessed to determine the best culturing conditions. We noted that advanced DMEM/F12 slowed the growth of PDT-LUAD#5 though it was passaged over ten times (depicted as *).