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. 2021 Apr 12;11:7904. doi: 10.1038/s41598-021-86784-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Passing-Bablok method comparison and correlation analysis for bile acids with average relative concentration higher than 0.01. The relative concentrations of Cholic Acid (A), Chenodeoxycholic Acid (B), Deoxycholic Acid (C) and Lithocholic Acid (D) in OMNIgene-GUT and snap frozen samples are plotted and indicated by the blue points. The snap frozen is taken as the reference method, while the OMNIgene-GUT is taken as the test method. The Passing-Bablok fit is shown as the red line. The allowable bias between the relative concentrations in OMNIgene-GUT and snap frozen samples is 30%.