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. 2021 Apr 12;11:7904. doi: 10.1038/s41598-021-86784-0

Table 1.

Bile Acid Concentration (pmol/mg) of each sample tested per donor.

Donor N° Storage container Sample ID Bile acid concentration (pmol/mg)
CA CDCA DCA LCA UDCA GCA GCDCA GDCA GLCA GUDCA TCA TCDCA TDCA TLCA TUDCA
Donor 1 OMNIgene-GUT D1-OMNIg-GUT_1 22.92 32.236 23.788 0.6908 0 0.0648 0.478 0 0 0 0.0408 0.0816 0 0 0.0012
D1-OMNIg-GUT_2 24.12 31.784 2.454 0.9 0 0.0476 0.4244 0 0 0 0.0284 0.0832 0 0 0.002
Snap frozen at −80 °C D1-SnapFrozen_1 206 21.2 15.2 0.8184 0 0.0876 0.0708 0 0 0 0.246 0.036 0.0168 0 0
D1-SnapFrozen_2 213.6 22.12 15.84 0.6676 0 0.1388 0.0748 0 0 0 0.238 0.0376 0.0244 0 0
Donor 2 OMNIgene-GUT D2-OMNIg-GUT_1 152.4 29 20.56 0.4924 1.652 19.776 27.952 0 0 0.0532 0.4396 0.6764 0 0 0.0156
D2-OMNIg-GUT_2 166.8 32 22.6 0.6576 1.794 22.348 29.308 0 0 0.0716 0.4568 0.7972 0 0 0.0148
Snap frozen at −80 °C D2-SnapFrozen_1 385.6 48 33.32 13.252 36.196 14.224 0.9752 0 0 0.0376 0.446 0.448 0 0 0.034
D2-SnapFrozen_2 548 78 52.4 0.5364 5.16 20.348 13.344 0 0 0.0544 0.3296 0.4364 0 0 0.044
Donor 3 OMNIgene-GUT D3-OMNIg-GUT_1 22.076 0.1596 110.4 86 0 10.688 18.092 11.412 0 0.106 18.236 0.9068 0.3808 0 0.062
D3-OMNIg-GUT_2 16.552 0.1332 91.2 61.6 0 0.9096 10.468 0.4844 0 0.08 11.816 0.6268 0.256 0 0.0372

Snap frozen at

−80 °C

 D3-SnapFrozen_1 31.556 0.03 98.4 204 12.944 0.0844 0.5056 0.5636 0.2836 0.0496 0.1656 0.3156 0.3196 0.156 0.046
D3-SnapFrozen_2 3.552 0 90.4 204 10.176 0.128 0.6068 0.6392 0.2492 0.0984 0.5336 0.5396 0.382 0.108 0.048
Donor 4 OMNIgene-GUT D4-OMNIg-GUT_1 0.8408 10.116 278.8 148.8 0 0.1452 0.7988 16.992 0 0.062 0.0404 0.0628 0.09 0 0
D4-OMNIg-GUT_2 0.9276 1.036 265.2 152 0 0.1388 0.8672 17.252 0 0.0356 0.0444 0.0592 0.082 0 0
Snap frozen at −80 °C D4-SnapFrozen_1 3.15 7.64 752 365.2 7.2 0.0584 0.1004 0.5824 0.194 0.0344 0 0.0084 0.0588 0 0
D4-SnapFrozen_2 14.476 2.074 596 351.2 39.876 0.0696 0.1032 0.6424 0.1152 0.0508 0 0.0056 0.042 0 0
Donor 5 OMNIgene-GUT D5-OMNIg-GUT_1 0.9956 1.134 83.2 134 0 0.8676 1.816 0.9612 0 0.0564 0.1436 0.2168 0.114 0.0124 0.006
D5-OMNIg-GUT_2 0.9584 10.256 81.2 128 0 0.7504 14.456 0.9028 0 0.056 0.1408 0.2056 0.1 0.0144 0.0064
Snap frozen at −80 °C D5-SnapFrozen_1 10.684 32.744 190.8 332 0 0.1704 0.4692 0.5532 0.1468 0.0344 0.1496 0.2536 0.2588 0.0632 0.0084
D5-SnapFrozen_2 20.968 0.9776 236 383.6 0 0.3192 0.4056 0.6488 0.2052 0.1336 0.662 0.2068 0.3836 0.06 0
Donor 6 OMNIgene-GUT D6-OMNIg-GUT_1 0.1608 0.2136 206.4 200.8 0 0.2296 0.7376 0.7228 0 0.046 0.0616 0.0884 0.0884 0.026 0
D6-OMNIg-GUT_2 0.7508 1.364 460 440 0 0.1016 0.124 0.5484 0 0.0924 0.2632 0.0968 0.2508 0.1104 0.0432
Snap frozen at −80 °C D6-SnapFrozen_1 0.7364 9.76 468 428 0 0.1264 0.1384 0.4332 0 0.1148 0.1656 0.0584 0.1948 0.1424 0
D6-SnapFrozen_2 0.7612 0.2624 178.8 193.6 0 0.2248 0.6368 0.6672 0 0.0476 0.0348 0.0776 0.0764 0.0152 0
Donor 7 OMNIgene-GUT D7-OMNIg-GUT_1 29.356 0.5664 408 157.2 0 0.7192 0.6348 37.236 0 0 0.1648 0.0604 0.2912 0 0
D7-OMNIg-GUT_2 29.956 0.53 408 150.4 0 0.7264 0.6436 3.848 0 0.0328 0.1876 0.066 0.3076 0 0.0116
Snap frozen at −80 °C D7-SnapFrozen_1 12.044 0 408 209.2 0 0.096 0.044 0.7248 0.2196 0 0 0.014 0.1508 0.042 0.0196
D7-SnapFrozen_2 0.9796 0 331.2 188.4 0 0.1696 0.0948 0.5992 0.1488 0 0.0332 0.0368 0.17 0.0516 0
Donor 8 OMNIgene-GUT D8-OMNIg-GUT_1 10.076 2.018 134 135.2 0 11.496 1.402 0.796 0 0.1288 0.2836 0.3304 0.1188 0 0.0192
D8-OMNIg-GUT_2 1.174 2.184 143.2 136.4 0.576 12.972 17.156 1.036 0 0.1432 0.3096 0.4008 0.1412 0 0.0168
Snap frozen at −80 °C D8-SnapFrozen_1 14.484 0.5604 338 364 0 0.2664 0.7544 0.5436 0 0.136 0.0936 0.2856 0.1176 0 0.0108
D8-SnapFrozen_2 26.372 3.206 273.6 317.6 15.684 0.3276 0.4696 0.272 0 0.0908 0.0592 0.12 0.0724 0 0

CA—Cholic Acid; CDCA—Chenodeoxycholic Acid; DCA—Deoxycholic Acid; LCA—Lithocholic Acid; UDCA—Ursodeoxycholic Acid; GCA—Glycocholic Acid; GCDCA- Glycochenodeoxycholic Acid; GDCA—Glycodeoxycholic Acid; GLCA—Glycolithocholic Acid; GUDCA—Glycoursodeoxycholic Acid; TCA—Taurocholic Acid; TCDCA—Taurochenodeoxycholic Acid; TDCA—Taurodeoxycholic Acid; TLCA—Taurolithocholic Acid; TUDCA—Tauroursodeoxycholic Acid.