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. 2021 Apr 12;12:2174. doi: 10.1038/s41467-021-22454-z

Fig. 4. Increased apoptosis of OCs and synovial macrophages derived from patients with late-stage RA by PRNPs.

Fig. 4

a Confocal images of the cellular uptake on OCs and synovial macrophages (n = 3 independent samples). Scale bar = 50 μm. b–d Quantitative analysis of the cellular uptake of C6-loaded NPs, RNPs or PRNPs on OCs (b), synovial macrophages (c), and HUVECs (d) after 1-h incubation at the C6 concentration of 50 ng/mL. Data represent mean ± SD (n = 3 independent samples). Statistical significance was determined by a two-sided Student’s t test. e, f Quantitative analysis for the apoptosis of OCs (e) and synovial macrophages (f) by CEL-NPs, CEL-RNPs, or CEL-PRNPs. Data represent mean ± SD (n = 3 independent samples). Statistical significance was determined by a two-sided Student’s t test. OCs osteoclasts, C6 coumarin 6, DAPI 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, MMP9 matrix metalloproteinase 9, HUVECs human umbilical vein endothelial cells, NPs poly (d, l-lactide-co-glycolide) (PLGA) nanoparticles, RNPs RGD peptide-modified PLGA nanoparticles, PRNPs MMP9-cleavable polyethylene glycol (PEG)- and RGD peptide-modified PLGA nanoparticles.