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. 2021 Apr 12;11:7928. doi: 10.1038/s41598-021-85119-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA expression of CXCL1 (A) and TNFα (B) in THP-1 cells incubated with SGBS-CM. THP-1 cells were incubated for 4 days with SGBS-CM from d4 (T1 or T2) and d8 (T3 or T4) of adipogenesis. The corresponding controls were ctrl#1: RPMI + unconditioned induction medium from d4 (1:1) and ctrl#2: RPMI + unconditioned differentiation medium from d8 (1:1). For qRT-PCR, mRNA samples of THP-1 macrophages were taken at d7 of differentiation. The TATA-Box binding protein (TBP) was used as a housekeeping gene. The data were statistically analyzed by one-way ANOVA on ranks with the Student–Newman–Keuls method. N = 6, n = 1 (4 pooled wells); ^ap < 0.05, ^aap < 0.01, ^aaap < 0.001, THP-1 + SGBS-CM [d4] vs. ctrl#1 or ctrl#2; ^bp < 0.05, ^bbp < 0.01, ^bbbp < 0.001, THP-1 + SGBS-CM [d4] vs. [d8].