Table 1.
Primers and sequences.
| Gene | Specificity | Sequence 5′ → 3' | Tm (°C) | Product size (bp) |
|---|---|---|---|---|
| hu GPER1 | fw | TTGGCACAGATCCTCGAACAA | 60 | 174 |
| rv | ATGAAACAGACACCCCAGCTC | |||
| hu ERα | fw | CAATGACTATGCTTCAGGCTAC | 60 | 198 |
| rv | CCACCTTTCATCATTCCCAC | |||
| hu CD14 | fw | GATTACATAAACTGTCAGAGGC | 58 | 143 |
| rv | TCCATGGTCGATAAGTCTTC | |||
| hu CD36 | fw | ACTGAGGACTGCAGTGTAGGA | 60 | 216 |
| rv | ACAAGCTCTGGTTCTTATTCACA | |||
| hu CD40 | fw | TATCAAAAAGGTGGCCAAG | 60 | 85 |
| rv | GAAGATCGTCGGGAAAATTG | |||
| hu CD206 | fw | AAATTTGAGGGCAGTGAAAG | 60 | 74 |
| rv | GGATTTGGAGTTTATCTGGTAG | |||
| hu CXCL1 | fw | AACATGCCAGCCACTGTGAT | 62 | 287 |
| rv | GCCCCTTTGTTCTAAGCCAG | |||
| hu HIF1α | fw | GAAACTACTAGTGCCACATC | 60 | 157 |
| rv | GGAACTGTAGTTCTTTGACTC | |||
| hu IL1β | fw | CATTGCTCAAGTGTCTGAAGC | 60 | 238 |
| rv | CATGGCCACAACAACTGACG | |||
| hu IL8 | fw | GGAGCACTCCATAAGGCACAA | 60 | 182 |
| rv | CAAAACTGCACCTTCACACAGA | |||
| hu MCP1 | fw | CCGAGAGGCTGAGACTAACC | 60 | 159 |
| rv | GGGGCATTGATTGCATCTGG | |||
| hu NAMPT | fw | CTAATGGCCTTGGGATTAAC | 58 | 114 |
| rv | TCCAGTGTAACAAAATTCCC | |||
| hu UCP1 | fw | ACAGCACCTAGTTTAGGAAG | 59 | 160 |
| rv | CTGTACGCATTATAAGTCCC | |||
| hu SIRT1 | fw | TTGGCACAGATCCTCGAACAA | 62 | 222 |
| rv | ATGAAACAGACACCCCAGCTC | |||
| hu TBP | fw | TGTGCTCACCCACCAACAAT | 60 | 199 |
| rv | AGTCGTCTTCCTGAATCCCT | |||
| hu TLR4 | fw | GGTCAGACGGTGATAGCGAG | 60 | 178 |
| rv | TTAGGAACCACCTCCACGCA | |||
| hu TNFα | fw | AGGCGCTCCCCAAGAAGACA | 60 | 153 |
| rv | TCCCTGGGGAACTCTTCCCTCT |