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. 2021 Feb 26;296:100481. doi: 10.1016/j.jbc.2021.100481

Figure 3.

Figure 3

Transmembrane protein 2 (TMEM2) is required for efficient cell migration on hyaluronan (HA)-containing substrates.A, representative images of the migration of TMEM2-depleted and control U2OS cells into a cell-free gap on mixed substrates of Col1 and HA. Top panels (HA15/0 h) show images of gaps immediately after removal of the ibidi 2-well Culture-Insert. Other panels show images of gaps after a 20 h incubation on Col1/HA15, Col1/HA150, and Col1/HA1500 substrates. The scale bar represents 200 μm. B, quantitative analysis of cell migration. Data represent the mean ± SD of the gap area covered by migratory cells relative to the area of the original gap (n = 15–24 per condition). ∗∗∗p < 0.001; n.s., not significant by two-way ANOVA with Bonferroni's multiple comparison test. C, confirmation of the specificity of siRNA effects. Migration assays were performed for parental U2OS cells treated with control siRNA (blue), parental U2OS cells treated with siRNA against human TMEM2 (red), and mouse TMEM2-transfected U2OS cells (mCherry-mTMEM2 cells) treated with siRNA against human TMEM2 (purple). Data represent mean ± SD (n = 9 per condition). ∗∗∗p < 0.001; n.s., not significant by two-way ANOVA with Bonferroni's multiple comparison test.