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. 2021 Feb 25;296:100469. doi: 10.1016/j.jbc.2021.100469

Figure 2.

Figure 2

hAPP does not exacerbate Drp1cKO-induced cell loss and morphologic changes.A, neuronal cell bodies labeled by NeuN staining in brain sections from 12-month-old mice. Hippocampi indicated by dotted outlines. Scale bar is 1 mm. B and C, Drp1 loss decreased both CA1 (B) and overall hippocampal (C) volume in 12-month-old mice. n = 4–5 mice/group (9–17 slices/mouse). Data are means ± S.E.M., ∗p < 0.05, ∗∗p < 0.01 by two-way ANOVA and Holm–Sidak post hoc test. D, Drp1cKO and hAPP Drp1cKO mice did not show any decrease in CA1 cell density at 12 months of age. n = 4–5 mice/group (4 slices/mouse). n.s. (not significant) by two-way ANOVA and Holm–Sidak post hoc test. E, Mitochondria in CA1 neurons in hippocampal slices from 6 to 7-month-old Drp1WT (control), Drp1cKO, hAPP-J20 (hAPP), and hAPP-J20 Drp1cKO (hAPP Drp1cKO) mice, identified by Tom20 immunofluorescence (green). Cell bodies (outer stippled outlines) were defined by Map2 staining. Scale bar is 4 μm. F, Drp1KO increased the proportion of cells with swollen mitochondria, while hAPP had no effect. n = 4 mice/group (3 slices/mouse). ∗p < 0.05 by Welch’s ANOVA and Games-Howell post hoc test (used instead of two-way ANOVA due to significant Levene’s test for equality of variance).