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. 2021 Feb 25;296:100469. doi: 10.1016/j.jbc.2021.100469

Figure 4.

Figure 4

Drp1 loss decreases MAMs in cultured neurons. Drp1KO and control neurons, with or without mutant hAPP, were cotransfected with reporters to visualize the ER (yellow, eYFP-ER) and mitochondria (red, mitoFarRed). A, three-dimensional reconstructions of confocal Z-stacks (rendered via max projection) showing neuronal cell bodies with MAMs identified by areas showing ER-mitochondria colocalization (cyan; with surface rendering). B, in the presence or absence of hAPP, Drp1KO cells showed fewer persistent MAMs (defined as contacts lasting 3–5, 6–8, or 9 min) than Drp1WT cells. C, Drp1KO decreased the total area of ER–mitochondria contacts (normalized to total mitochondrial volume) with or without hAPP. D and E, in the most stable contacts (lasting 9 min), Drp1KO reduced MAM number and total MAM area in the presence and absence of hAPP expression. (B–E), hAPP alone had no significant effect on MAMs as compared with control. n = 8–11 coverslips/group (with 11–12 cells/group), compilation of three experiments. Data show mean ± SEM; p = 0.064, ∗p < 0.05, ∗∗p < 0.01, n.s. (not significant), by Welch’s ANOVA and Games-Howell post hoc test. Scale bars are 5 μm.