Figure 5.

hAPP expression increases evoked cytosolic calcium in the cell body of neurons, but drastically decreases evoked calcium in the absence of Drp1.A and B, Drp1KO and control neurons, with or without mutant hAPP, were cotransfected with the cytosolic calcium (cytCa²⁺) sensor GCaMP6f (81) and subjected to electrical stimulation (30 Hz for 3 s (blue vertical bars) and 10 Hz for 60 s (horizontal blue bar)). Drp1KO alone had no significant effect on the amplitude of evoked cytCa²⁺, whereas hAPP expression increased cytCa²⁺ in the presence of Drp1 and decreased cytCa²⁺ in the absence of Drp1. n = 7–8 coverslips/group (with 17–60 cells/group), compilation of three experiments. Data are representative traces normalized to baseline and control (A) and means ± S.E.M. (B) ∗p < 0.05 Drp1KO versus hAPP Drp1KO, ∗∗p < 0.01 control versus hAPP, ∗∗∗∗p < 0.0001 hAPP versus hAPP Drp1KO by two-way ANOVA and Holm–Sidak test. Control versus Drp1KO was not significant (n.s.).