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. 2021 Mar 16;24(4):102315. doi: 10.1016/j.isci.2021.102315

Figure 1.

Figure 1

FAM134B engages ATG9A on the ER membrane.

(A) Schematic view of the predicted topology of FAM134B.

(B and C) (B) Insertion of the consensus sequence for N-glycosylation within the aa140–208 region of FAM134B did not consistently result in glycosylation. (C) Inset in (B). N-glyco, N-glycosylated FAM134B; no-glyco, non-glycosylated FAM134B.

(D and E) Immunoprecipitation of FAM134B pulled down only the immature and ER-based form of ATG9A. (E) Inset in (D). m, mature/N-glycosylated ATG9A; im, immature ATG9A.

(F) Imaris reconstruction of SIM showing interaction of Atg9a (cyan) and Fam134b (green). The ER (red) was labeled with ER3-mCherry. Interacting Atg9a and Fam134b are shown in gray.