Figure 1.

MAIT cells in the thymus and lung in the absence of Bcl11b at steady state

(A) Representative histograms of Bcl11b in TCRβ+MR1t-5-OP-RU+ MAIT cells. Thymic developmental stages (left), liver and lung (right).

(B) Bcl11b MFI in MAIT cells in thymic developmental stages and lung (n = 4).

(C) Representative dot plots of MR1t-5-OP-RU and control MR1t-6-FP staining in thymi of Bcl11b^F/F/PLZFcre and Bcl11b^F/F (WT) mice.

(D) Frequencies of TCRβ+MR1t-5-OP-RU+ MAIT cells from thymi of Bcl11b^F/F/PLZFcre and Bcl11b^F/F (WT) mice (n = 5). The data from thymus are derived from 10 pairs of mice in which two FACS files from the same type of mice (KO or WT) were concatenated.

(E) Representative dot plot of YFP and CD44 in TCRβ+MR1t-5-OP-RU+ MAIT cells isolated from thymi of Bcl11b^F/F/PLZFcre/R26R-EYFP mice.

(F and G) Representative dot plots (F) and frequencies (G) of Bcl11b KO and WT TCRβ+MR1t-5-OP-RU+ MAIT cell developmental stages in thymi of Bcl11b^F/F/PLZFcre and WT mice (n = 4). The data are derived from 8 pairs of mice in which two FACS files from the same type of mice (KO or WT) were concatenated.

(H) Representative dot plots of MR1t-5-OP-RU and control MR1t-6-FP staining in lung of BCL11B^F/F/PLZFcre and BCL11B^F/F (WT) mice.

(I and J) Frequencies (I) and absolute numbers (J) of TCRβ+MR1t-5-OP-RU+ MAIT cells in lung of Bcl11b^F/F/PLZFcre and WT mice. Each dot represents the data from one mouse.

(K) Representative dot plot of YFP and CD44 in TCRβ+MR1t-5-OP-RU+ MAIT cells isolated from the lung of Bcl11b^F/F/PLZFcre/R26R-EYFP mice at steady state.

Data are represented as mean ± SEM. Student's t-test was used for statistical analysis. p < 0.05 was considered significant.