Figure 4.

Bcl11b KO MAIT cells fail to expand and produce IL17A during Salmonella infection but have elevated granzyme levels

In all experiments Bcl11b^F/F/PLZFcre and control mice were infected with 10⁶ cfu of Salmonella enterica ser. Typhimurium BRD509 (Salmonella BRD509) intranasally. Mice were euthanized on day 7 post infection.

(A) Representative dot plots of lin- TCRβ+ MR1t-5-OP-RU and control MR1t-6-FP staining in lung of infected Bcl11b^F/F/PLZFcre and WT mice.

(B) Frequencies and absolute numbers of lin- TCRβ+ MR1t-5-OP-RU+ MAIT cells in lung of infected Bcl11b^F/F/PLZFcre and WT mice.

(C and D) Representative dot plots (C), frequencies and MFI (D) of Ki67+ CD44+ lung Bcl11b KO and WT lin-TCRβ+MR1t-5-OP-RU+ MAIT cells from infected mice.

(E and F) Representative dot plots (E), frequencies and absolute numbers (F) of Bcl11b KO and WT RORγt+ IL17A+ lin-TCRβ+MR1t-5-OP-RU+ MAIT cells from lung of infected mice.

(G) Representative dot plots of RORγt and Tbet within lin-TCRβ+MR1t-5-OP-RU+ in the lung of Bcl11b^F/F/PLZFcre and WT infected mice.

(H) Frequencies of Bcl11b KO and WT RORγt+ Tbet-, RORγt-Tbet+, RORγt+Tbet+ and RORγt-Tbet-lin-TCRβ+MR1t-5-OP-RU+ MAIT cell subsets in the lung of Bcl11b^F/F/PLZFcre and WT infected mice.

(I–L) Representative dot plots (I, K) and frequencies (J, L) of GzmB+ CD44+ (I, J) and GzmA+ CD44+ (K, L) lin-TCRβ+MR1t-5-OP-RU+ MAIT cells in the lung of infected Bcl11b^F/F/PLZFcre and WT mice.

Data are represented as mean ± SEM. Statistics were conducted as paired t test. p < 0.05 was considered significant.