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. 2021 Mar 30;9:661116. doi: 10.3389/fcell.2021.661116

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Copyright © 2021 Deng, Yan, Dai, Chen, Qu, Wu and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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N-cadherin knockdown promoted the odontogenic differentiation of dental pulp stem cells (DPSCs). DPSCs transfected with a control or N-cadherin-specific shRNA lentivirus were subjected to odontogenic induction for 7 or 14 days. The expression level of odontogenic-related genes (DSPP and Runx2) were detected by qPCR and western blotting on day 7 and 14 (A,B). The alkaline phosphatase (ALP) activity was measured by ALP staining on day 7 (C). The formation of mineralized nodules was detected by alizarin red staining (ARS) on day 14 (D). Scale bars = 500 μm. Data are presented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.