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. 2021 Mar 30;9:661116. doi: 10.3389/fcell.2021.661116

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Copyright © 2021 Deng, Yan, Dai, Chen, Qu, Wu and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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N-cadherin downregulation enhanced β-catenin activity and odontogenic differentiation in vivo. Dental pulp stem cells transfected with a control or N-cadherin-specific shRNA lentivirus were loaded onto the β-tricalcium phosphate scaffold, and transplanted subcutaneously into immunocompromised nude mice for 4 weeks (A). The samples were subjected to hematoxylin and eosin (H&E) staining (B), Masson’s trichrome staining (C), DSPP, nestin and β-catenin immunohistochemistry (IHC; D–F, respectively). The numbers of DSPP, nestin and β-catenin positive cells were quantified (G–I, respectively). Scale bars = 200 μm. Data are presented as the mean ± SD (n = 5 per group). ***P < 0.001.