TABLE 1.

Inherent variations in oxygen diffusion distances (culture medium height) due to the dimensions of typically utilized plastic cell culture systems.

Culture system	Culture surface area (cm2)	Typical culture medium volume range (mL)	Culture medium height range (mm)
96-well	0.32	0.1–0.2	3.12–6.25
24-well	1.9	0.5–1	2.63–5.26
6-well	9.6	1–3	1.04–3.13
T-75 flask	75	8–15	1.07–2
T-175 flask	175	35–53	2–3.03

1D oxygen diffusion from the air/liquid interface varies from 1.04 to 6.25 mm depending on dimensions and culture medium volume.