TABLE 3.
Summary of previously identified secondary metabolites in the extracts of the three different Xenorhabdus cultures using high resolution UPLC-MS and MS analysis, as well as genetic analysis to confirm group identity. Refer to Figures 2–4; Table 2 for UPLC-MS and Supplementary Figures S1, S2 and S5 for high resolution MS analyses and structures.
|
Nr |
Experimental M r | Proposed Identity | Theoretical M r | Mass error (ppm) | Comment/Reference | ||
|---|---|---|---|---|---|---|---|
| Protonated | Sodiated | Protonated | Sodiated | ||||
| - | 1050.7719 | - | PAX5 | 1050.7766 | - | 4 | Low concentration, Fuchs et al. (2011) |
| - | 1052.7937 | - | PAX1′ | 1052.7923 | - | −1 | Low concentration, Fuchs et al. (2011) |
| - | 1066.8075 | - | PAX3′ | 1066.8079 | - | 0.4 | Low concentration, Fuchs et al. (2011) |
| 8 | 1078.8058 | - | PAX7E | 1078.8079 | - | 2 | Low concentration, Dreyer et al. (2019) |
| 18 | 407.2171 | - | Xenocoumacin II | 407.2182 | - | 3 | Reimer et al. (2011), Dreyer et al. (2019) |
| 34 | 1286.8077 | 1308.7849 | Xenoamicin D1 | 1286.8077 | 1308.7901 | 0, 4 | Novel Xenoamicin? Zhou et al. (2013), this study |
| 35, 36 | 1300.8235 | 1322.8052 | Xenoamicin A1,2,3 | 1300.8238 | 1322.8058 | 0.2, 0.5 | Novel Xenoamicins? Zhou et al. (2013), this study |
| 37 | 1330.8326 | - | Xenoamicin B2 +OH, x | 1330.8344 | - | 1 | Novel Xenoamicin, Zhou et al. (2013), this study |
| 38, 39 | 1328.8552 | 1350.8311 | Xenoamicin B1 (V→I/L) | 1328.8552 | 1350.8371 | 0, 4 | Novel Xenoamicin, Zhou et al. (2013), this study |
| 42, 44 | 1314.8390 | 1336.8177 | Xenoamicin B2 (I→V, I/L→V) | 1314.8395 | 1336.8215 | 0.4, 3 | Novel Xenoamicin, Zhou et al. (2013), this study |