Figure 1.

SFV-AM6 shows enhanced oncolytic potency in GL261 cells in vitro

(A) Schematic presentation of the SFV-AM6 genome. (B) Representative images of plaques produced by SFV4 and SFV-AM6 in the BHK-21 and GL261 cell lines. (C) Analysis of cell attachment (cell index) with the xCELLigence system (mean ± SD). GL261 cells infected with SFV4 (blue), SFV-AM6 (red) at an MOI of 0.01, or left uninfected (black) (n = 5) are shown. The time point of infection is marked with a black arrowhead. (D) Cell viability of infected GL261 cells was measured with an MTS assay (mean ± SD) 72 h after infection using various MOIs. (E) Caspase-3/7 activity (mean ± SD) in GL261 cells 16 h after infection at an MOI of 1. (F) Virus titers measured (mean ± SD) by plaque titration 24 h after infection at an MOI of 0.01. (G) qRT-PCR analysis of interferon (IFN)-β and indicated IFN-stimulated genes (ISGs) in GL261 cells, 16 h after infection at an MOI of 1 with SFV4 (blue) or SFV-AM6 (red). Data are plotted as mean ± SD. IFIT1, IFN-induced protein with tetratricopeptide repeats 1; TNF, tumor necrosis factor; CXCL10, C-X-C motif chemokine 10; PD-L1; programmed death-ligand 1. Statistical analysis was performed by a Student’s two-tailed, unpaired t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.