Figure 2.

SFV-AM6 infection of GL261 cells triggers phagocytosis and maturation in co-cultured dendritic cells (DCs)

(A and B) Flow cytometry analysis of DC phagocytic activity. (A) Percentage of pHrodo reagent-positive DCs (CD11c⁺) after co-culture with noninfected or SFV-AM6-infected GL261 cells. (B) Analysis of direct GL261-GFP cell phagocytosis by DCs. Percentage of GFP⁺CD11c⁺ DCs after co-culture with noninfected or SFV-AM6-infected GL261-GFP cells. (C–F) Mean fluorescence intensity (MFI) of DC maturation markers, CD80 (C), CD86 (D), CD40 (E), and MHC class II (F) on DCs (CD11c^high/CD11b⁺ cells) co-cultured with SFV-AM6-infected GL261 cells. Noninfected GL261 cells or SFV-AM6 virus alone was used as a control. Data are plotted as mean ± SD. Statistical analysis was done by a two-tailed, unpaired t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.