Figure 4.

Intraperitoneally injected SFV-AM6-124T increases immune cell infiltration in orthotopic GL261 gliomas

(A) Mouse treatment scheme. (B) Kaplan-Meier plot of GL261-bearing mice either treated with SFV-AM6-124T (n = 26), anti-PD1 (n = 7), SFV-AM6-124T + anti-PD1 combination (n = 16), or left untreated (n = 24). (C–F) Flow cytometry analysis of CD45⁺ cells that were isolated from the brains of GL261-bearing mice after different treatments (n = 6). Absolute counts of CD45^high cells (C), CD3⁺ cells (D), CD8⁺ T cells (E), CD4⁺ T cells (F), CD3⁺CD4⁻CD8⁻ double-negative (DN) T cells (G), B cells (H), DCs (I), granulocytes (J), CD11b+ myeloid cells (K), and NK cells (L). Data are plotted as mean ± SD. Statistical analysis was done by one-way ANOVA with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.