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. 2021 Mar 30;14:643763. doi: 10.3389/fnmol.2021.643763

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Copyright © 2021 Schlabitz, Monni, Ragot, Dipper-Wawra, Onken, Holtkamp and Fidzinski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activity and time dependent c-Fos gene expression in rat slices. Induction of c-Fos mRNA relative to basal gene expression in (A) Hippocampus, (B) Entorhinal cortex, and (C) Temporal cortex, respectively. All investigated conditions are represented: control slices (control), 4-AP treated slices (4-AP) with experimental duration of 1, 2, 3 or 4 h. For relative quantification in RT-qPCR, Ywhaz was used as reference gene. Scatter plots show means of individual rats (n = 20) since tissue was pooled before RT-qPCR, superimposed lines represent median ± interquartile range (*p < 0.05, ***p < 0.001).