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Chromatogram of the elution step of wtHbF and mutants αA12D, αA19D, and αA12D/A19D on a HiTrap Q HP column. The column was equilibrated with 20 mM Tris-HCl pH 7.4 and the dashed line shows the change in conductivity (mS/cm) as the NaCl concentration was increased over a linear gradient.
