Figure 3.

DNA cleavage of 3 ng/μl pUC18 plasmids incubated with 75 μM Hb of the different HbF mutants at 37°C in 20 mM phosphate buffer pH 7.2. The graph shows the mean values of the remaining DNA content, with error bars representing the standard deviation, together with the fitted curves of the exponential decay (dashed lines). Statistical differences were determined by independent t-test (p < 0.05). The pictures of the gel bands show the supercoiled band over time.