Figure 5.
The crystal structure of pdb ID 4MQJ (Soman and Olson, 2013) and four 3D models of the recombinant HbFs generated by the rigid-body docking. The α-subunits are displayed in blue and the γ-subunits in green, while the heme groups are red. Below, the chain-chain diagrams calculated by ChimeraX software are shown for the different structures generated by the rigid-body docking (SASREF, contact condition 7 Å). From left to right: crystal structure wtHbF, in solution SAXS of wtHbF, αA12D, αA19D, and αA12D/A19D. Blue dots are the α-chains, and green dots are the γ-chains. The solid lines show major interface areas ≥300 Å2, and the dotted lines show minor interface areas.