FIGURE 1.

Identification of microexons in Drosophila by TopHat. (A) Identified reliable microexons from RNA-seq data of a variety of Drosophila samples by TopHat. Orange: novel microexons; blue: annotated microexons in Flybase. (B) Length distribution of the identified microexons by TopHat. (C) Validation of novel microexons by RT-PCR. Each microexon was validated by amplification of cDNA using primers located in its flanking exons. Lengths and numbering of microexons and the genes to which they belong are indicated. Blank boxes: flanking exons, orange boxes: microexons. Primers information is listed in Supplementary Table 1.