Figure 4.

ScRNA-seq analysis of IPF, SSc-ILD, and control plasmacytoid dendritic cell populations. (A) UMAP plot of the reclustered original B lymphocyte and plasma cell cluster, identified by cell type. (B) UMAP plot of the reclustered original B lymphocyte and plasma cell cluster, identified by disease status. (C) Expression of IL3RA, CLEC4C, CLIC3, and NRP1, identifying pDCs. (D) Expression of IFNAR1and IFNAR2 in IPF versus SSc-ILD pDCs. (E) Pathways upregulated in SSc-ILD pDCs compared to IPF pDCs. scRNA-seq, single-cell RNA-sequencing; IPF, idiopathic pulmonary fibrosis; SSc-ILD, systemic sclerosis-associated interstitial lung disease; UMAP, uniform manifold approximation and projection; pDCs, plasmacytoid dendritic cells.