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. 2021 Mar 30;9:650961. doi: 10.3389/fbioe.2021.650961

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Copyright © 2021 Burgardt, Moustafa, Persicke, Sproß, Patschkowski, Risse, Peters-Wendisch, Lee and Wendisch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of ubiA expression on pHBA production by the strain UBI400 (pRG_Duet2-ddsA-ubiA). (A) Pathway from the precursor FPP toward the intermediate 10P-HB with heterologously expressed ddsA and ubiA marked in blue and green, respectively. (B) The expression of ubiA was induced or not induced via addition of ATc either with or without the additional induction of ddsA expression via addition of IPTG. The concentration of pHBA was measured in the respective supernatants. IPP, isopentenyl diphosphate; FPP, farnesyl diphosphate; DPP, decaprenyl diphosphate; DPP, decaprenyl diphosphate; 10P-HB, 3-decaprenyl-4-hydroxybenzoate; ddsA, decaprenyl diphosphate synthase from P. denitrificans; ubiA, 4-hydroxybenzoate octaprenyltransferase from E. coli.