FIGURE 6.

Verification of ubi gene function and expression. (A) E. coli deletion mutants were transformed with control vectors and complementation vectors (bold) and inoculated into M9 minimal medium with 20 mM succinate as sole carbon source. Complementation enabled growth after 45/48 h as can be compared with the positive control strain K-12. (B) SDS–PAGE of UBI000 crude extracts carrying different vectors for expression analysis. Confirmed expression based on theoretical protein weights is depicted in green, missing bands are shown in gray at the expected position.