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. 2021 Mar 30;9:650961. doi: 10.3389/fbioe.2021.650961

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Copyright © 2021 Burgardt, Moustafa, Persicke, Sproß, Patschkowski, Risse, Peters-Wendisch, Lee and Wendisch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High performance liquid chromatography (HPLC) and LC-MS analyses of quinone extracts from different UBI strains for CoQ10 detection. (A) Overlaid chromatograms of the CoQ10 standard and UBI strains with UBI413 (red) carrying the vectors pRG_Duet2-ddsA-ubiA, pEC-XT99A-ubiDIBX, and pEKEx3-ubiGHEF to facilitate CoQ10 production. (B) Isotope pattern of CoQ10 from quinone extract of UBI413 (red) and from the CoQ10 standard (black). (C) Growth curves of strains that were used for quinone extraction. (D) Base peak chromatogram of m/z 880.71, the ammonium adduct mass of CoQ10 in the quinone extract of UBI413 (red) and in the CoQ10 standard (black).