FIGURE 8.

Batch fermentation of C. glutamicum UBI413 in 2-L scale. The cultivation was performed in CGXII medium with 40 g L^–1 glucose, supplemented with 0.5 g L^–1 of L-methionine, antibiotics, 1 mM of IPTG and 0.25 μg mL⁻¹ of ATc for induction of gene expression at 30°C, pH 7.0 and an aeration rate of 2 NL min⁻¹. The rDOS was maintained at 30% by control of agitator frequency. Glucose concentration (green) and OD₆₀₀ (black) are shown in squares, rDOS (red) and agitator frequency (blue) are indicated by lines, production of pHBA is shown in triangles. An upper estimate of CoQ10 production is given in the results.