TABLE 1.
Strains used in this work.
| Strains | Description | Source |
| C. glutamicum | ||
| WT | Wild type, ATCC 13032 | ATCC |
| WT (pVWEx1) | WT carrying pVWEx1 | This work |
| WT (pVWEx1-ddsA) | WT carrying pVWEx1-ddsA | This work |
| WT (pVWEx1-ADS) | WT carrying pVWEx1-ADS | This work |
| ΔcrtOPΔidsAΔcrtB2I’I2 | WT carrying deletion of crtOP (cg0717-cg0723), idsA (cg2384), and crtB2I’I2 (cg2668-cg2672) | Henke et al., 2018 |
| UBI000 | LP4::Ptuf-ispA mutant of C. glutamicum ΔcrtOPΔidsAΔcrtB2I’I2 | This work |
| UBI000 (pVWEx1-ADS) | UBI000 carrying pVWEx1-ADS | This work |
| UBI000 (pEC-XT99A) | UBI000 carrying pEC-XT99A | This work |
| UBI000 (pEC-XT99A-ubiDIBX) | UBI000 carrying pEC-XT99A-ubiDIBX | This work |
| UBI000 (pEKEx3) | UBI000 carrying pEKEx3 | This work |
| UBI000 (pEKEx3-ubiGHEF) | UBI000 carrying pEKEx3-ubiGHEF | This work |
| UBI100 | ΔpobA mutant of UBI000 | This work |
| UBI200 | ΔpcaHG::Psod-ubiCFBR mutant of UBI100 | This work |
| UBI300 | Δvdh::PilvC-aroGFBR mutant of UBI200 | This work |
| UBI400 | ΔqsuABCD::Ptuf-qsuC mutant of UBI300 | This work |
| UBI401 | UBI400 carrying pVWEx4 | This work |
| UBI405 | UBI400 carrying pRG_Duet2-ddsA-ubiA | This work |
| UBI412 | UBI400 carrying pRG_Duet2-ddsA-ubiA and pEC-XT99A-ubiDIBX | This work |
| UBI413 | UBI400 carrying pRG_Duet2-ddsA-ubiA, pEC-XT99A-ubiDIBX and pEKEx3-ubiGHEF | This work |
| E. coli | ||
| K-12 | K-12 MG1655 wild type, ATCC 47076 | ATCC |
| DH5α | F-thi-1 endA1 hsdr17(r-, m-) supE44 1lacU169 (Φ80lacZ1M15) recA1 gyrA96 | Hanahan, 1983 |
| S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | Simon et al., 1983 |
| ΔubiG | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ–, ΔubiG785::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| ΔubiG (pEKEx3) | ΔubiG carrying pEKEx3 | This work |
| ΔubiG (pEKEx3-ubiG) | ΔubiG carrying pEKEx3-ubiG | This work |
| ΔubiH | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ–, ΔubiH758::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| ΔubiH (pEKEx3) | ΔubiH carrying pEKEx3 | This work |
| ΔubiH (pEKEx3-ubiG) | ΔubiH carrying pEKEx3-ubiG | This work |
| ΔubiH (pEKEx3-ubiGH) | ΔubiH carrying pEKEx3-ubiGH | This work |
| ΔubiE | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ–, ΔubiE778::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| ΔubiE (pEKEx3) | ΔubiE carrying pEKEx3 | This work |
| ΔubiE (pEKEx3-ubiGH) | ΔubiE carrying pEKEx3-ubiGH | This work |
| ΔubiE (pEKEx3-ubiGHEF) | ΔubiE carrying pEKEx3-ubiGHEF | This work |
| ΔubiF | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ–, ΔubiF722::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al., 2006 |
| ΔubiF (pEKEx3) | ΔubiF carrying pEKEx3 | This work |
| ΔubiF (pEKEx3-ubiGH) | ΔubiF carrying pEKEx3-ubiGH | This work |
| ΔubiF (pEKEx3-ubiGHEF) | ΔubiF carrying pEKEx3-ubiGHEF | This work |