TABLE 2.
Plasmids used in this work.
| Plasmids | Description | Source |
| pVWEx1 | KanR, Ptac, lacIq, pHM1519 oriVCg, C. glutamicum/E. coli expression shuttle vector | Peters-Wendisch et al., 2001 |
| pVWEx1-ddsA | KanR, pVWEx1 overexpressing ddsA from P. denitrificans | This work |
| pVWEx1-ADS | KanR, pVWEx1 overexpressing ADS from A. annua | This work |
| pSH1-ispA | KanR, Ptuf. pHM1519 oriVCg, C. glutamicum/E. coli expression shuttle vector overexpressing ispA from E. coli | This work |
| pRG_Duet2 | KanR, Ptac, lacIq, PtetR/tetA, tetR, pBL1 oriVCg, dual-inducible C. glutamicum/E. coli expression shuttle vector | Gauttam et al., 2019 |
| pRG_Duet2-ddsA-ubiA | KanR, pRG_Duet2 overexpressing ddsA from P. denitrificans (induced by IPTG) and ubiA from E. coli (induced by ATc) | This work |
| pEKEx3 | SpecR, Ptac, lacIq, pBL1 oriVCg, C. glutamicum/E. coli expression shuttle vector | Stansen et al., 2005 |
| pEKEx3-ubiG | SpecR, pEKEx3 overexpressing ubiG from E. coli | This work |
| pEKEx3-ubiGH | SpecR, pEKEx3 overexpressing ubiG and ubiH from E. coli | This work |
| pEKEx3-ubiGHEF | SpecR, pEKEx3 overexpressing ubiG, ubiH, ubiE and ubiF from E. coli | This work |
| pEC-XT99A | TetR, Ptrc, lacIq, pGA1 oriVCg, C. glutamicum/E. coli expression shuttle vector | Kirchner and Tauch, 2003 |
| pEC-XT99A-ubiDIBX | TetR, pEC-XT99A overexpressing ubiD, ubiI, ubiB and ubiX from E. coli | This work |
| pK19mobsacB | KmR, pK19 oriVEc, sacB, lacZα, E. coli/C. glutamicum shuttle vector for construction of insertion and deletion mutants in C. glutamicum | Schäfer et al., 1994 |
| pK19mobsacB-LP4::Ptuf-ispA | pK19mobsacB with a construct for insertion of ispA from E. coli K-12 under control of C. glutamicum promoter Ptuf into CgLP4 | This work |
| pK19mobsacB-ΔpobA | pK19mobsacB with a construct for deletion of pobA (cg1226), which has been amplified from APS529 Purwanto et al., 2018 with the primers pobA-fw and pobA-rv | This work |
| pK19mobsacB-ΔpcaHG::Psod-ubiCFBR | pK19mobsacB with a construct for deletion of pcaHG (cg2631-cg2630) and insertion of ubiCL31A from E. coli K-12 under control of C. glutamicum promoter Psod | Purwanto et al., 2018 |
| pK19mobsacB-Δvdh::PilvC-aroGFBR | pK19mobsacB with a construct for deletion of vdh (cg2953) and insertion of aroGD146N from E. coli K-12 under control of C. glutamicum promoter PilvC, which has been amplified from APS529 Purwanto et al., 2018 with the primers vdh-fw and vdh-rv | This work |
| pK19mobsacB-ΔqsuABCD::Ptuf-qsuC | pK19mobsacB with a construct for deletion of qsuABCD (cg0501-cg0504) and insertion of qsuC (cg0503) under control of C. glutamicum promoter Ptuf | Walter et al., 2020 |