TABLE 3.
Primers used in this work.
| Primers | Sequence (5′ to 3′) |
| pVW-ddsA-fw / pRG-ddsA-fw | CCTGCAGGTCGACTCTAGAGGAAAGGAGGCCCTTCAGATGGGCATGAACGAAAACGT |
| pVW-ddsA-rv / pRG-ddsA-rv | GAGCTCGGTACCCGGGGATCTCAGGACAGGCGCGAGACGA |
| pRG-ubiA-fw | AGAGGAGGAAAGGGTATACGGAAAGGAGGCCCTTCAGATGGAGTGGAGTCTGACGCA |
| pRG-ubiA-rv | CAATTTAAATCCTAGGGCTATCAGAAATGCCAGTAACTCA |
| pG-ubiG-fw | CCTGCAGGTCGACTCTAGAGGTATACGAAAGGAGGCCCTTCAGATGAATGCCGAAAAATCGC |
| pG-ubiG-rv | GAGCTCGGTACCCGGGGATCTCACTTATTCTGCGTGTGC |
| pGH-ubiG-rv | GTAAGTCACTTATTCTGCGTGTGC |
| pGH-ubiH-fw | GCACACGCAGAATAAGTGACTTACCCGCGGGAAAGGAGGCCCTTCAGATGAGCGTAATCATCGTCGGTGG |
| pGH-ubiH-rv | GAGCTCGGTACCCGGGGATCTCAACGCGCCACCCAACC |
| pGHEF-ubiH-rv | AAGAGTCAACGCGCCACCCAACC |
| pGHEF-ubiE-fw | GGTTGGGTGGCGCGTTGACTCTTCCATGGGAAAGGAGGCCCTTCAGATGGTGGATAAGTCACAAG |
| pGHEF-ubiE-rv | GTGACTCAGAACTTATAACCACGATGC |
| pGHEF-ubiF-fw | GCATCGTGGTTATAAGTTCTGAGTCACGCTAGCGAAAGGAGGCCCTTCAGATGACAAATCAACCAACGGAAATTGC |
| pGHEF-ubiF-rv | GAGCTCGGTACCCGGGGATCCTACAACCCTAACGCATATTTCAG |
| pDIBX-ubiD-fw | ACACAGGAAACAGACCATGGGCGGCCGCGAAAGGAGGCCCTTCAGATGGACGCCATGAAATATAAC |
| pDIBX-ubiD-rv | GTAAGTCAGGCGCTTTTACCGTTG |
| pDIBX-ubiI-fw | CAACGGTAAAAGCGCCTGACTTACACTAGTGAAAGGAGGCCCTTCAGATGCAAAGTGTTGATGTAGC |
| pDIBX-ubiI-rv | AAGAGTTAACGCAGCCATTCAGG |
| pDIBX-ubiB-fw | CCTGAATGGCTGCGTTAACTCTTGTTTAAACGAAAGGAGGCCCTTCAGATGACGCCAGGTGAAGTACG |
| pDIBX-ubiB-rv | GTGACTCAGCGTGTTTTGCGCCAAC |
| pDIBX-ubiX-fw | GTTGGCGCAAAACACGCTGAGTCACGCGATCGCGAAAGGAGGCCCTTCAGATGAAACGACTCATTGTAGGCATCAG |
| pDIBX-ubiX-rv | CCGGGTACCGAGCTCGAATTTTATGCGCCCTGCCAGC |
| LP4-US-fw | CCTGCAGGTCGACTCTAGAGCCGTTCGGCTGACTCCTTC |
| LP4-US-rv | CATTCGCAGGGTAACGGCCACATCAAAAAATCCGCCGTTCCTTG |
| Ptuf-ispA-fw | CAAGGAACGGCGGATTTTTTGATGTGGCCGTTACCCTGCGAATG |
| ispA-Term-rv | CTTCCGCATCCAAACTCACTTAGTCAAAAGAGTTTGTAGAAACGCAAAAAGG |
| LP4-DS-fw | CCTTTTTGCGTTTCTACAAACTCTTTTGACTAAGTGAGTTTGGATGCGGAAG |
| LP4-DS-rv | GAGCTCGGTACCCGGGGATCCTCACTAGTACGCGGATAAATG |
| LP4-conf-fw | TCCGCTGATTGCAGATGGTC |
| LP4-conf-rv | GCTCCGACACAGAGTCAATG |
| pobA-fw | CCTGCAGGTCGACTCTAGAGAAATGCGGTGGTCCAGGCGTAGC |
| pobA-rv | GAGCTCGGTACCCGGGGATCCCAACCAAAGCCGTCGATAAGGAA |
| pobA-conf-fw | AAGGCCTGGTGTGAGTGCGTGAAA |
| pobA-conf-rv | GTACGGCACCTGCGATGAAC |
| pca-conf-fw | GGCGTACGTACATCAGTGGA |
| pca-conf-rv | CCCACTGCGGATCAAAAAGG |
| vdh-fw | CCTGCAGGTCGACTCTAGAGTCGAGGAACACCACGTTGTGG |
| vdh-rv | GAGCTCGGTACCCGGGGATCATTATTTGGCTGCTCTTCCTCAG |
| vdh-conf-fw | GCACTTCCCGGAGGCTACCA |
| vdh-conf-rv | TGCATCTGCTGCAACGGTGG |
| qsu-conf-fw | GTTCGTGGACAAGTGTGGTGG |
| qsu-conf-rv | CTACCGCGCGGATTAAACC |
Ribosomal binding sites are in bold, and binding regions of Gibson primers are underlined.