Figure 1.

T cell – neutrophil co-culture system. Schematic of culture system. Rapid isolation of peripheral blood neutrophils by negative magnetic selection is performed before autologous T cells are isolated. Cells are co-cultured at a 5: 1 Neutrophil: T cell ratio either without any stimulation (‘naïve’) or in the presence of CD3/CD28 stimulating activation cocktail (‘early’). T cells are also cultured alone in the presence of αCD3 αCD28-coated Dynabeads. 24 hours later Dynabeads are removed and fresh autologous neutrophils isolated. These are co-cultured without any further stimulation (‘late’).