Skip to main content
. 2021 Mar 30;12:633486. doi: 10.3389/fimmu.2021.633486

Figure 2.

Figure 2

Neutrophil contact differentially affects early- and late-stage activating T cells. T cells and neutrophils were isolated from peripheral blood of healthy human donors using negative magnetic separation, and were cultured together at a 5:1 neutrophil: T cell ratio. (A) representative flow cytometry plot and (B–D) quantification of proliferation of CD4+ and CD8+ T cells alone and in the presence of resting neutrophils. (E–G) Following 24 hours co-culture T cell activation was assessed by flow cytometry analysis of PD-1 and (H–J) cell culture supernatant was collected at 24 hours and cytokine production assessed by ELISA. In all cases red symbols = naïve T cells, black = early-activating and blue = late activating. # = two samples were below the limit of detection; ## = all samples were below the limit of detection. N values of individual donors, each of which was plated separately: (B, C) 7; (D) 3; (F, G) naïve 4, early 10 for CD4 and 7 for CD8, late 7 for CD4 and 8 for CD8 (H) naïve 3, early 13, late 10; (I) naïve 4, early 13, late 12; (J) early 4 late 3. Data between T cells exposed to neutrophils and control T cells were analyzed by paired t tests on raw data before conversion to percentages.